A8200

Sigma

 

Aminopeptidase from Aeromonas proteolytica

lyophilized powder, 50-150 units/mg protein

CAS Number:37288-67-8
Enzyme Commission (EC) Number:3.4.11.10   ( BRENDA | IUBMB )
EC Number:232-874-6
MDL number:MFCD00166325

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Description

General descriptionA zinc-containing enzyme.
SpecificityCatalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Unit DefinitionOne unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.
Physical formLyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Properties

formlyophilized powder
compositionProtein, ~40% biuret
foreign activityendopeptidase, essentially free (as measured by the gelatin digestion assay of Prescott and Wilkes)
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany1

References

referencePrescott, J.M. and Wilkes, S.H. Meth. Enzymol. 45, 530, (1976) Abstract